黃溫雅
開授課程
大學部
上學期︰普臨床血液學實驗、臨床血液學實習、細胞遺傳學實習
下學期︰醫學遺傳學
碩士班
上學期︰高等分子生物學
下學期︰DNA損害修復與疾病
MyLab實驗室研究方向
1.肝癌發生(hepato-carcinogenesis)與B型肝炎病毒感染之關係與分子機轉: B型肝炎病毒感染是導致肝癌很重要的原因。在癌症發生過程中，細胞常發生基因不穩定性(genomic instability)。因此維持基因的穩定與完整性對於預防癌症發生扮演重要的角色。我們發現B型肝炎病毒的表面抗原pre-S2突變種會造成基因不穩定性及造成DNA損害(DNA damage)。因此我們以B型肝炎病毒為study model，探討慢性B型肝炎帶原者在轉變成肝癌的過程中DNA damage與DNA repair機制的調控及其對HCC發生的影響。
2.DNA Repair 之分子病理: DNA Repair (DNA修復)是細胞內維持DNA結構完整及修復DNA損害最重要的一個機制。當生物體DNA受到環境途變原(environmental mutagens)例如x-ray，紫外線，以及有毒化學物質等刺激而引起DNA結構不正常時，就需靠DNA Repair 功能來使之恢復正常功能。DNA Repair 功能異常的病人由於DNA突變率較高，導致癌症的機率較正常人高出許多。我們主要的研究方向為探討DNA Repair的分子機轉及其調控，尤其是近年來才分離出的因子hHR23 A及hHR23B (human homolog of Rad23 A / B)，探討其在DNA Repair過程中所扮演的角色。除此之外，我們也利用微矩陣(Microarray)生物晶片技術來探討紫外線等環境途變原所引發的DNA Repair分子機轉。同時我們也進一步分析DNA Repair不正常病人的細胞株，以了解DNA Repair異常所引發的相關病理機制。
指導學生
〔助理〕
|
姓名 |
研究主題 |
|---|---|
|
李○萍 |
Development of a serological detection method for hepatitis B virus large surface antigen. |
|
陳○玉 |
Association of the oncofetal Nei endonuclease VIII-like 3 protein with hepatitis B virus-induced cirrhosis. |
〔博士後研究員〕
|
姓名 |
研究主題 |
|---|---|
|
張○媖 |
The interactive mechanism of hepatitis B virus surface protein and importinα1 and the effect of this complex for DNA damage in hepatocarcinogenesis. |
〔碩士班〕
|
姓名 |
論文題目 |
|---|---|
|
艾○琳(醫技所二年級） |
Development of hepatitis B virus large surface antigen pre-S2 mutant DNA and protein detection method in chronic carriers. |
|
林○酉(醫技所二年級） |
Inhibition of integrin-mediated adhesion and migration of hepatoma cells by disintegrins. |
|
蘇○雅(醫技所一年級） |
|
過去成員
〔已畢業博士〕
|
姓名 |
研究主題 |
|---|---|
|
謝○璇(基醫所)-2007畢 |
Genomics instability induced by HBV Pre-S mutant surface proteins |
〔已畢業碩士〕
|
姓名 |
論文題目 |
|---|---|
|
賴○婷 (分醫所)-2002畢 |
The protein interaction studies of the two human nucleotide excision repair factors hHR23A and hHR23B and their roles as protein degradation regulators |
|
蔡○和 (醫工所)-2002畢 |
Application of cDNA microarray on studies of UVC-induced cell’s gene expressions |
|
謝○璇 (醫技所)-2003畢 |
Oxidative stress and genomic instabilities caused by the HBV pre-S mutant surface antigens |
|
黃○忻 (分醫所) -2003畢 |
Functional studies of the human hHR23A and hHR23B proteins in DNA repair and proteolysis |
|
謝○娟 (醫工所) -2004畢 |
Functional studies of DNA repair genes hHR23A and hHR23B |
|
王○閔 (醫技所)-2005畢 |
Genetic polymorphisms associated with breast cancer in south Taiwan |
|
蔡○霓 (醫技所)-2005畢 |
Studies of antioxidant activities in Agaricus blazei Murill extracts |
|
王○婷 (分醫所)-2006畢 |
Association of mitochondrial function with chemo-resistance in lung cancer cells |
|
許○斐 (醫技所)-2006畢 |
Screening and functional studies of human proteins which interact with Streptococcal pyogenic exotoxin B (SPE B) |
|
董○蕙 (分醫所)-2007畢 |
Screening and functional studies of human proteins which interact with DNA-damage binding protein (DDB2) |
|
黃○君 (醫技所)- 2007畢 |
Functional studies of HBV surface antigen pre-S2 mutation with its association of JAB1 and KPNA2 |
|
李○陵 (醫技所)-2009畢 |
Association of human 8-oxoguanine DNA glycosylase 1 isoform with NDUFB10 and its potential role in mitochondrial DNA repair |
|
蘇○虹(醫技所)-2010畢 |
Functional studies of the α- and β-hydroxyguanine glycosylases in mitochondrial DNA repair |
|
阮玉○兒(醫技所)-2010畢 |
Microsatellite instability caused by pre-S2 mutant LHBS |
|
劉○傑 (醫技所)-2010畢 |
Genomic instability in hepatitis B virus-induced hepatoma in transgenic mice. |
|
高○齡 (醫技所)-2011畢 |
Studies of the killing effects of HDAC inhibitor sulforaphane to hepatoma cell lines. |
|
李○萍 (醫技所)-2012畢 |
Functional characterization of the human base excision repair factor nth endonuclease III-like 1. |
|
李○翰 (醫技所)-2012畢 |
Development of a serological detection method for hepatitis B virus large surface antigen. |
|
陳○玉 (醫技所)-2013畢 |
Association of the oncofetal Nei endonuclease VIII-like 3 protein with hepatitis B virus-induced cirrhosis. |
照片
指導學生之特殊榮譽
無相關資料
榮譽及獎勵
-
Poster award. Hsieh, Y. H., Su, I.-J., Lei, H.-Y., Lai, M.-D., Chang, W.-T. and Huang, W. (corresponder), 2003 Genomic stress and mutations caused by pre-S2 mutant hepatitis B virus surface antigen. The 18th Biomedical Conference, Taipei.
-
Research Grant Award. Hsieh, Y.-H. and Huang, W. 2004. Study of pre-S HBsAg mutants in genomic instability. Chinese Hepatitis Prevention Foundation. Taiwan.
-
Research Grant Award. Hsieh, H.-C. and Huang, W. 2007. Hepatitis B virus large surface antigen pre-S2 mutant on DNA repair. Chinese Hepatitis Prevention Foundation. Taiwan.
-
Excellence Research Award, 2009. Title: Streptococcal pyrogenic exotoxin B cleaved human S-adenosylhomocysteine hydrolase and induced hypermethioninemia. J. Infectious Diseases. Cheng-Hsin Medical Foundation, Taiwan.
-
Research Award, 2013. Title: Histone deacetylase inhibitor suberoylanilide hydroxamic acid suppresses the pro-oncogenic effects induced by hepatitis B virus pre-S2 mutant oncoprotein and represents a potential chemopreventive agent in high-risk chronic HBV patients. Taiwan Society of Laboratory Medicine.
-
Poster award. Chang, Y.-Y., Hsieh, Y.-H., and Huang, W., 2013. Hepatitis B virus pre-S2 mutant surface protein inhibits Nijmegen breakage syndrome 1-mediated DNA repair and induces genomic instability in hepatocarcinogenesis. HBV Molecular Biology Annual Meeting, Shanghai, China.