黃溫雅
黃溫雅 特聘教授 Wen-Ya Huang
辦公室電話：5766
辦公室地點：生醫卓群大樓八樓13823室
實驗室電話：5767
實驗室地點：生醫卓群大樓八樓13807室
學術成大：黃 溫雅
Publons：Wen-Ya Huang
開授課程
大學部
上學期︰普臨床血液學實驗、臨床血液學實習、細胞遺傳學實習
下學期︰醫學遺傳學
碩士班
上學期︰高等分子生物學
下學期︰DNA損害修復與疾病
MyLab實驗室研究方向
1. 肝癌發生 (hepato-carcinogenesis) 與 B 型肝炎病毒感染之關係與分子機轉: B 型肝炎病毒感染是導致肝癌很重要的原因。在癌症發生過程中，細胞常發生基因不穩定性(genomic instability)。因此維持基因的穩定與完整性對於預防癌症發生扮演重要的角色。我們發現 B 型肝炎病毒的表面抗原 pre-S2 突變種會造成基因不穩定性及造成 DNA 損害(DNA damage)。因此我們以 B 型肝炎病毒為 study model，探討慢性 B 型肝炎帶原者在轉變成肝癌的過程中 DNA damage 與 DNA repair 機制的調控及其對 HCC 發生的影響。
2. DNA Repair 之分子病理: DNA Repair (DNA修復) 是細胞內維持 DNA 結構完整及修復 DNA 損害最重要的一個機制。當生物體 DNA 受到環境途變原(environmental mutagens) 例如 x-ray，紫外線，以及有毒化學物質等刺激而引起 DNA 結構不正常時，就需靠 DNA Repair 功能來使之恢復正常功能。DNA Repair 功能異常的病人由於 DNA 突變率較高，導致癌症的機率較正常人高出許多。我們主要的研究方向為探討 DNA Repair 的分子機轉及其調控，尤其是近年來才分離出的因子 hHR23 A 及 hHR23B (human homolog of Rad23 A / B)，探討其在 DNA Repair 過程中所扮演的角色。除此之外，我們也利用微矩陣(Microarray) 生物晶片技術來探討紫外線等環境途變原所引發的 DNA Repair 分子機轉。同時我們也進一步分析 DNA Repair 不正常病人的細胞株，以了解 DNA Repair 異常所引發的相關病理機制。
指導學生
〔助理〕
| 姓名 | 研究主題 |
|---|---|
| 李○萍 | Development of a serological detection method for hepatitis B virus large surface antigen. |
| 陳○玉 | Association of the oncofetal Nei endonuclease VIII-like 3 protein with hepatitis B virus-induced cirrhosis. |
〔博士後研究員〕
| 姓名 | 研究主題 |
|---|---|
| 張○媖 | The interactive mechanism of hepatitis B virus surface protein and importinα1 and the effect of this complex for DNA damage in hepatocarcinogenesis. |
〔碩士班〕
| 姓名 | 論文題目 |
|---|---|
| 艾○琳 (醫技所二年級） | Development of hepatitis B virus large surface antigen pre-S2 mutant DNA and protein detection method in chronic carriers. |
| 林○酉 (醫技所二年級） | Inhibition of integrin-mediated adhesion and migration of hepatoma cells by disintegrins. |
| 蘇○雅 (醫技所一年級） |
過去成員
〔已畢業博士〕
| 姓名 | 研究主題 |
|---|---|
| 謝○璇 (基醫所)-2007畢 | Genomics instability induced by HBV Pre-S mutant surface proteins |
〔已畢業碩士〕
| 姓名 | 論文題目 |
|---|---|
| 賴○婷 (分醫所)-2002畢 | The protein interaction studies of the two human nucleotide excision repair factors hHR23A and hHR23B and their roles as protein degradation regulators |
| 蔡○和 (醫工所)-2002畢 | Application of cDNA microarray on studies of UVC-induced cell’s gene expressions |
| 謝○璇 (醫技所)-2003畢 | Oxidative stress and genomic instabilities caused by the HBV pre-S mutant surface antigens |
| 黃○忻 (分醫所) -2003畢 | Functional studies of the human hHR23A and hHR23B proteins in DNA repair and proteolysis |
| 謝○娟 (醫工所) -2004畢 | Functional studies of DNA repair genes hHR23A and hHR23B |
| 王○閔 (醫技所)-2005畢 | Genetic polymorphisms associated with breast cancer in south Taiwan |
| 蔡○霓 (醫技所)-2005畢 | Studies of antioxidant activities in Agaricus blazei Murill extracts |
| 王○婷 (分醫所)-2006畢 | Association of mitochondrial function with chemo-resistance in lung cancer cells |
| 許○斐 (醫技所)-2006畢 | Screening and functional studies of human proteins which interact with Streptococcal pyogenic exotoxin B (SPE B) |
| 董○蕙 (分醫所)-2007畢 | Screening and functional studies of human proteins which interact with DNA-damage binding protein (DDB2) |
| 黃○君 (醫技所)- 2007畢 | Functional studies of HBV surface antigen pre-S2 mutation with its association of JAB1 and KPNA2 |
| 李○陵 (醫技所)-2009畢 | Association of human 8-oxoguanine DNA glycosylase 1 β isoform with NDUFB10 and its potential role in mitochondrial DNA repair |
| 蘇○虹 (醫技所)-2010畢 | Functional studies of the α- and β-hydroxyguanine glycosylases in mitochondrial DNA repair |
| 阮玉○兒 (醫技所)-2010畢 | Microsatellite instability caused by pre-S2 mutant LHBS |
| 劉○傑 (醫技所)-2010畢 | Genomic instability in hepatitis B virus-induced hepatoma in transgenic mice. |
| 高○齡 (醫技所)-2011畢 | Studies of the killing effects of HDAC inhibitor sulforaphane to hepatoma cell lines. |
| 李○萍 (醫技所)-2012畢 | Functional characterization of the human base excision repair factor nth endonuclease III-like 1. |
| 李○翰 (醫技所)-2012畢 | Development of a serological detection method for hepatitis B virus large surface antigen. |
| 陳○玉 (醫技所)-2013畢 | Association of the oncofetal Nei endonuclease VIII-like 3 protein with hepatitis B virus-induced cirrhosis. |
照片
指導學生之特殊榮譽
無相關資料
榮譽及獎勵
Poster award. Hsieh, Y. H., Su, I.-J., Lei, H.-Y., Lai, M.-D., Chang, W.-T. and Huang, W. (corresponder), 2003 Genomic stress and mutations caused by pre-S2 mutant hepatitis B virus surface antigen. The 18th Biomedical Conference, Taipei.
Research Grant Award. Hsieh, Y.-H. and Huang, W. 2004. Study of pre-S HBsAg mutants in genomic instability. Chinese Hepatitis Prevention Foundation. Taiwan.
Research Grant Award. Hsieh, H.-C. and Huang, W. 2007. Hepatitis B virus large surface antigen pre-S2 mutant on DNA repair. Chinese Hepatitis Prevention Foundation. Taiwan.
Excellence Research Award, 2009. Title: Streptococcal pyrogenic exotoxin B cleaved human S-adenosylhomocysteine hydrolase and induced hypermethioninemia. J. Infectious Diseases. Cheng-Hsin Medical Foundation, Taiwan.
Research Award, 2013. Title: Histone deacetylase inhibitor suberoylanilide hydroxamic acid suppresses the pro-oncogenic effects induced by hepatitis B virus pre-S2 mutant oncoprotein and represents a potential chemopreventive agent in high-risk chronic HBV patients. Taiwan Society of Laboratory Medicine.
Poster award. Chang, Y.-Y., Hsieh, Y.-H., and Huang, W., 2013. Hepatitis B virus pre-S2 mutant surface protein inhibits Nijmegen breakage syndrome 1-mediated DNA repair and induces genomic instability in hepatocarcinogenesis. HBV Molecular Biology Annual Meeting, Shanghai, China.
