開授課程

• 大學部
  • 上學期
    • 臨床血液學實驗
    • 臨床血液學實習
    • 細胞遺傳學實習
  • 下學期
    • 醫學遺傳學
• 碩士班
  • 上學期
    • 高等分子生物學
  • 下學期
    • DNA損害修復與疾病

MyLab 

1.肝癌發生(hepato-carcinogenesis)與B型肝炎病毒感染之關係與分子機轉: B型肝炎病毒感染是導致肝癌很重要的原因。在癌症發生過程中，細胞常發生基因不穩定性(genomic instability)。因此維持基因的穩定與完整性對於預防癌症發生扮演重要的角色。我們發現B型肝炎病毒的表面抗原pre-S2突變種會造成基因不穩定性及造成DNA損害(DNA damage)。因此我們以B型肝炎病毒為study model，探討慢性B型肝炎帶原者在轉變成肝癌的過程中DNA damage與DNA repair機制的調控及其對HCC發生的影響。

2.DNA Repair 之分子病理: DNA Repair (DNA修復)是細胞內維持DNA結構完整及修復DNA損害最重要的一個機制。當生物體DNA受到環境途變原(environmental mutagens)例如x-ray，紫外線，以及有毒化學物質等刺激而引起DNA結構不正常時，就需靠DNA Repair 功能來使之恢復正常功能。DNA Repair 功能異常的病人由於DNA突變率較高，導致癌症的機率較正常人高出許多。我們主要的研究方向為探討DNA Repair的分子機轉及其調控，尤其是近年來才分離出的因子hHR23 A及hHR23B (human homolog of Rad23 A / B)，探討其在DNA Repair過程中所扮演的角色。除此之外，我們也利用微矩陣(Microarray)生物晶片技術來探討紫外線等環境途變原所引發的DNA Repair分子機轉。同時我們也進一步分析DNA Repair不正常病人的細胞株，以了解DNA Repair異常所引發的相關病理機制。

 

 

 

 

 

 

指導學生

〔助理〕

姓名	研究主題
李○萍	Development of a serological detection method for hepatitis B virus large surface antigen.
陳○玉	Association of the oncofetal Nei endonuclease VIII-like 3 protein with hepatitis B virus-induced cirrhosis.

〔博士後研究員〕

姓名	研究主題
張○媖	The interactive mechanism of hepatitis B virus surface protein and importinα1 and the effect of this complex for DNA damage in hepatocarcinogenesis.

〔碩士班〕

姓名	論文題目
艾○琳(醫技所二年級）	Development of hepatitis B virus large surface antigen pre-S2 mutant DNA and protein detection method in chronic carriers.
林○酉(醫技所二年級）	Inhibition of integrin-mediated adhesion and migration of hepatoma cells by disintegrins.
蘇○雅(醫技所一年級）

過去成員

〔已畢業博士〕

姓名	研究主題
謝○璇(基醫所)-2007畢	Genomics instability induced by HBV Pre-S mutant surface proteins

〔已畢業碩士〕

姓名	論文題目
賴○婷 (分醫所)-2002畢	The protein interaction studies of the two human nucleotide excision repair factors hHR23A and hHR23B and their roles as protein degradation regulators
蔡○和 (醫工所)-2002畢	Application of cDNA microarray on studies of UVC-induced cell’s gene expressions
謝○璇 (醫技所)-2003畢	Oxidative stress and genomic instabilities caused by the HBV pre-S mutant surface antigens
黃○忻 (分醫所) -2003畢	Functional studies of the human hHR23A and hHR23B proteins in DNA repair and proteolysis
謝○娟 (醫工所) -2004畢	Functional studies of DNA repair genes hHR23A and hHR23B
王○閔 (醫技所)-2005畢	Genetic polymorphisms associated with breast cancer in south Taiwan
蔡○霓 (醫技所)-2005畢	Studies of antioxidant activities in Agaricus blazei Murill extracts
王○婷 (分醫所)-2006畢	Association of mitochondrial function with chemo-resistance in lung cancer cells
許○斐 (醫技所)-2006畢	Screening and functional studies of human proteins which interact with Streptococcal pyogenic exotoxin B (SPE B)
董○蕙 (分醫所)-2007畢	Screening and functional studies of human proteins which interact with DNA-damage binding protein (DDB2)
黃○君 (醫技所)- 2007畢	Functional studies of HBV surface antigen pre-S2 mutation with its association of JAB1 and KPNA2
李○陵 (醫技所)-2009畢	Association of human 8-oxoguanine DNA glycosylase 1  isoform with NDUFB10 and its potential role in mitochondrial DNA repair
蘇○虹(醫技所)-2010畢	Functional studies of the α- and β-hydroxyguanine glycosylases in mitochondrial DNA repair
阮玉○兒(醫技所)-2010畢	Microsatellite instability caused by pre-S2 mutant LHBS
劉○傑 (醫技所)-2010畢	Genomic instability in hepatitis B virus-induced hepatoma in transgenic mice.
高○齡 (醫技所)-2011畢	Studies of the killing effects of HDAC inhibitor sulforaphane to hepatoma cell lines.
李○萍 (醫技所)-2012畢	Functional characterization of the human base excision repair factor nth endonuclease III-like 1.
李○翰 (醫技所)-2012畢	Development of a serological detection method for hepatitis B virus large surface antigen.
陳○玉 (醫技所)-2013畢	Association of the oncofetal Nei endonuclease VIII-like 3 protein with hepatitis B virus-induced cirrhosis.

照片

榮譽及獎勵

• Poster award. Hsieh, Y. H., Su, I.-J., Lei, H.-Y., Lai, M.-D., Chang, W.-T. and Huang, W. (corresponder), 2003 Genomic stress and mutations caused by pre-S2 mutant hepatitis B virus surface antigen. The 18th Biomedical Conference, Taipei.
• Research Grant Award. Hsieh, Y.-H. and Huang, W. 2004. Study of pre-S HBsAg mutants in genomic instability. Chinese Hepatitis Prevention Foundation. Taiwan.
• Research Grant Award. Hsieh, H.-C. and Huang, W. 2007. Hepatitis B virus large surface antigen pre-S2 mutant on DNA repair. Chinese Hepatitis Prevention Foundation. Taiwan.
• Excellence Research Award, 2009. Title: Streptococcal pyrogenic exotoxin B cleaved human S-adenosylhomocysteine hydrolase and induced hypermethioninemia. J. Infectious Diseases. Cheng-Hsin Medical Foundation, Taiwan.
• Research Award, 2013. Title: Histone deacetylase inhibitor suberoylanilide hydroxamic acid suppresses the pro-oncogenic effects induced by hepatitis B virus pre-S2 mutant oncoprotein and represents a potential chemopreventive agent in high-risk chronic HBV patients. Taiwan Society of Laboratory Medicine.
• Poster award. Chang, Y.-Y., Hsieh, Y.-H., and Huang, W., 2013. Hepatitis B virus pre-S2 mutant surface protein inhibits Nijmegen breakage syndrome 1-mediated DNA repair and induces genomic instability in hepatocarcinogenesis. HBV Molecular Biology Annual Meeting, Shanghai, China.

技術轉移 

• 「篩檢B型肝炎病毒pre-S2區域缺失之基因晶片與蛋白質以及其臨床應用」技術轉移至普生股份有限公司（201503~203003）。
• Chuang, W.-J., Fu, W.-M., Huang, T.-F., Huang, W., Tang, C.-H., and Chen, C.-Y., 2008. “Disintegrin variants and pharmaceutical uses thereof, USA Patent Application Publication (20080188413A1)”. Technology transfer to Anchen Pharmaceuticals (Taiwan, inc.)