Education

• Ph.D., Life Science, National Defense Medical Center, Taiwan
• M.S., Biological Science and Technology, National Chiao Tung University, Taiwan
• B.S., Medical Technology, National Cheng Kung University, Taiwan

Research

Glycosylation plays a crucial role manipulating post-translational modification of more than 90% of proteins in eukaryotic cells. Glycans expressed on cell surface have participated in many recognition phenomena by interacting with their corresponding proteins or receptors. Hence, our research interests focus on exploring interactions between glycan binding proteins with specific oligosaccharides. We have developed two high-throughput carbohydrate microarray platforms and applied to investigate glycan binding profiles of lectins, antibodies, proteins, as well as viral particles. The carbohydrate recognition preferences of influenza clinical isolates and Enterovirus 71 are also surveyed. In addition, we are interested in the relationship between tumorigenesis with the regulation and expression of glycosyltransferases, as well as cell surface glycan structures. The carbohydrate tumor markers of oral cancer and colon cancer initiating cells are now investigated.

Selected publications: (Since 2013)

• Chen HP, Lee YK, Huang SY, Shih PC, Hsu PC, Chang CF . Phthalate exposure promotes chemotherapeutic drug resistance in colon cancer cells. Oncotarget. 2018, 9(17), 13167-13180. (*Corresponding author). (5.168, 44/217, Oncology)
• Wang LC, Yao HW, Chang CF , Wang SW, Wang SM and Chen SH. Suppression of interleukin-6 increases enterovirus A71 lethality in mice. J Biomed Sci. 2017, 24(1):94. (2.8, 51/128, Medicine, Research, and experimental)
• Guu SY, Lin TH, Chang SC, Wang RJ, Hung LY, Fang PJ, Tang, WC, Yu P, Chang CF . Serum N-glycome characterization and anti-carbohydrate antibody profiling in oral squamous cell carcinoma patients. PLoS One. 2017, 12:e0178927. (*Corresponding author). (2.806, 15/64, Multidisciplinary sciences)
• Lin WL, Chen CC, Shi GY, Ma CY, Chang CF , Wu HL. Monocytic thrombomodulin promotes cell adhesion through interacting with its ligand, Lewisy. Immunol Cell Biol. 2017, 95:372-379. (*Corresponding author). (4.557, 38/150, Immunology)
• Cheung SK, Chuang PK, Huang HW, Hwang-Verslues WW, Cho CH, Yang WB, Shen CN, Hsiao M, Hsu TL, Chang CF , Wong CH. Stage-specific embryonic antigen-3 (SSEA-3) and beta3GalT5 are cancer specific and significant markers for breast cancer stem cells. Proc Natl Acad Sci U S A. 2016; 113:960-965. (9.423, 4/63, Multidisciplinary sciences)
• Lin WL, Guu SY, Tasi CC, Prakash E, Viswaraman M, Chen HB, Chang CF . Derivation of cinnamon blocks leukocyte attachment by interacting with sialosides. PLoS One. 2015;10: e0130389. (*Corresponding author). (3.057, 11/63, Multidisciplinary sciences)
• Lin WL, Lin YS, Shi GY, Chang CF , Wu HL. Lewisy promotes migration of oral cancer cells by glycosylation of epidermal growth factor receptor. PLoS One. 2015;10: e0120162. (*Corresponding author). (3.057, 11/63, Multidisciplinary sciences)
• Su PY, Wang YF, Huang SW, Lo YC, Wang YH, Wu SR, Shieh DB, Chen SH, Wang JR, Lai MD, Chang CF . Cell surface nucleolin facilitates enterovirus 71 binding and infection. J Virol. 2015;89: 4527-4538. (*Corresponding author). (4.606, 5/33, Virology)
• Chen CK, Yang CY, Hua KT, Ho MC, Johansson G, Jeng YM, Chen CN, Chen MW, Lee WJ, Su JL, Lai TC, Chou CC, Ho BC, Chang CF , Lee PH, Chang KJ, Hsiao M, Lin MT, Kuo ML. Leukocyte cell-derived chemotaxin 2 antagonizes MET receptor activation to suppress hepatocellular carcinoma vascular invasion by protein tyrosine phosphatase 1B recruitment. Hepatology. 2014;59: 974-985. (11.711, 4/78, Gastroenterology & hepatology)
• Lee YK, Lin TH, Chang CF , Lo YL. Galectin-3 silencing inhibits epirubicin-induced ATP binding cassette transporters and activates the mitochondrial apoptosis pathway via beta-catenin/GSK-3beta modulation in colorectal carcinoma. PLoS One. 2013;8: e82478. (*Corresponding author). (3.057, 11/63, Multidisciplinary sciences)
• Chen PK, Chang BI, Kuo CH, Chen PS, Cho CF, Chang CF , Shi GY, Wu HL. Thrombomodulin functions as a plasminogen receptor to modulate angiogenesis. FASEB J. 2013;27: 4520-4531. (5.299, 7/86, Biology)
• Lin WL, Chang CF , Shi CS, Shi GY, Wu HL. Recombinant lectin-like domain of thrombomodulin suppresses vascular inflammation by reducing leukocyte recruitment via interacting with Lewis Y on endothelial cells. Arterioscler Thromb Vasc Biol. 2013;33: 2366-2373. (5.969, 4/63, Peripheral vascular disease)
• Chen YT, Ho CL, Chen PK, Chen YL, Chang CF . Anterior gradient 2: a novel sensitive tumor marker for metastatic oral cancer. Cancer Lett. 2013;339: 270-278. (*Corresponding author) (5.992, 23/213, Oncology)
• Chen YT, Chong YM, Cheng CW, Ho CL, Tsai HW, Kasten FH, Chen YL, Chang CF . Identification of novel tumor markers for oral squamous cell carcinoma using glycoproteomic analysis. Clin Chim Acta. 2013;420: 45-53. (*Corresponding author) (2.799, 7/30, Medical laboratory technology)


• All Refereed Papers