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Hua-Lin Wu(Professor)


Lab place: Medical college of NCKU 9F

Academic experience
  • 1968 ~ 1972 ---- Bachelor of Science, National Taiwan University, ROC.
  • 1972 ~ 1974 ---- Master degree, National Taiwan University, ROC.
  • 1976 ~ 1980 ---- Ph. D, Ohio State University, USA.
  • 1980 ~ 1983 ---- Research Fellow, Northwestern University, USA.
  • 1983 ~ 1988 ---- Associate Professor, Department of Biochemistry & Molecular biology, Colloege of Medicin, National Cheng Kung University
  • 1988 ~ 2010 ---- Professor of Department of Biochemistry & Molecular biology,National Cheng Kung University
  • 1989 ~ 1995 ---- Director of Department of Medical Technology, National Cheng Kung University
  • 1996 ~ 1999 ---- Director of Department of Biochemistry & Molecular biology, National Cheng Kung University
  • 2002 ~ present ---- Distinguished Professor of Department of Biochemistry & Molecular biology, National Cheng Kung University
  • 2010 ~ present ---- Chair Professor of Department of Biochemistry & Molecular biology, National Cheng Kung University

Research Interest

Cardiovascular disease is still an important global health problem. Vascular Biology is the major research interest in my lab. Thrombomodulin is a transmembrane protein in vascular endothelial cells and keratinocytes. It can regulate blood coagulation by activating protein C, which in turn inhibits blood coagulation by inactivation of factors Va and VIIIa. Thrombomodulin also plays an important role in cell-cell adhesion. It can regulate the distribution of plasminogen and migration of endothelial cells. Recently we also demonstrated that it can regulate inflammation responses by association with CD14 and monocyte adhesion to inflammatory endothelial cells. We also interested in the role of thrombomodulin in wound healing and in vascular diseases including atherosclerosis and aortic aneurysm using tissue specific gene knockout mice and recombinant proteins.


Expertise

VASCULAR BIOLOGY、HAEMOSTASIS AND FIBRINOLYSIS、BIOTECHNOLOGY


Selected Awards
  • The 54th Academic Award for Biology and Medical and Agronomy Sciences, Ministry of Education, Taiwan (2010)
  • The 10th Y.Z. HSU Scientific Award (2012)
  • The 16th National Chair Professorship Award, Ministry of Education, Taiwan (2013)

    • Selected Publications
  • Lai, C.-H., Wang, K.-C., Kuo, C.-H., Lee, F.-T., Cheng, C.-L., Chang, B.-I., Yang, Y.-J., Shi, G.-Y., Wu, H.-L. (2017) Recombinant adeno-associated virus vector carrying the thrombomodulin lectin-like domain for the treatment of abdominal aortic aneurysm. Atherosclerosis, 262 ,62-70.
  • Lin, W.-L., Chen, C.-C., Shi, G.-Y., Ma, C.-Y., Chang, C.-F. and Wu, H.-L. (2017). Monocytic thrombomodulin promotes cell adhesion through interacting with its ligand, Lewisy. Immunology and Cell Biology, 95: 372–379.
  • Cheng, T.-L., Lai, C.-H., Shieh, S.-J., Jou, Y.-B., Yeh, J.-L., Yang, A.-L., Wang, Y.-H., Wang, C.-Z., Chen, C.-H., Shi, G.-Y., Ho, M.-L., Wu, H.-L. (2016). Myeloid thrombomodulin lectin-like domain inhibits osteoclastogenesis and inflammatory bone loss. Sci Rep, 6:28340.
  • Hsu, Y.-Y., Shi, G.-Y., Wang, K.-C., Ma, C.-Y., Cheng, T.-L., Wu, H.-L. (2016). Thrombomodulin promotes focal adhesion kinase activation and contributes to angiogenesis by binding to fibronectin. Oncotarget, 7:68122-68139.
  • Lai, C. –H., Wang, K. –C., Lee, F. –T., Tsai, H. –W., Ma, C. –Y., Cheng, T. –L., Chang, B. –I., Yang, Y. –J., Shi, G. –Y., and Wu, H. –L. (2016) Toll-like receptor 4 is essential in the development of abdominal aortic aneurysm. PLoS One. 11:e0146565.
  • Wang, K. -C., Li, Y. -H., Shi, G. -Y., Tsai, H. -W., Luo, C. -Y., Cheng, M. -H., Ma C. -Y., Hsu, Y. -Y., Cheng, T. -L., Chang B. -I., Lai, C. -H., and Wu, H. -L. (2015) Membrane-bound thrombomodulin regulates macrophage inflammation in abdominal aortic aneurysm. Arterioscler Thromb Vasc Biol. 35: 2412-2422.
  • Cheng, T. -L., Lai, C. -H., Chen, P. -K., Cho, C. -F., Hsu, Y. -Y., Wang, K. -C., Lin, W. -L., Chang, B. -I., Liu, S. -K., Wu, Y.-T., Hsu, C. -K., Shi, G. -Y., Wu, H. -L. (2015). Thrombomodulin promotes diabetic wound healing by regulating toll-like receptor 4 expression. J Invest Dermatol, 135:1668-1675.
  • Huang, Y. -H., I, C. -C., Kuo, C. -H., Hsu, Y. -Y., Lee, F. -T., Shi, G. -Y., Tseng, S. -H., Wu, H. -L. (2015). Thrombomodulin promotes corneal epithelial wound healing. PLoS One, 10:e0122491.
  • Kuo, C. -H., Chang, B. -I., Lee, F. -T., Chen, P. -K., Lee, J. -S., Shi, G. -Y., Wu, H. -L. (2015). Development of recombinant AAV2/8 carrying kringle domains of human plasminogen for sustained expression and cancer therapy. Hum Gene Ther, 26: 603-613.
  • Kuo, C. -H., Sung, M. -C., Chen, P. -K., Chang, B. -I., Lee, F. -T., Cho, C. -F., Hsieh, T. -T., Huang, Y. -C., Li, Y. -H., Shi, G. -Y., Luo, C. -Y., Wu, H. -L. (2015). FGFR1 mediates recombinant thrombomodulin domain-induced angiogenesis. Cardiovasc Res, 105:107-117.
  • Lin, W. -L., Lin, Y. -S., Shi, G. -.Y, Chang, C. -F., Wu, H. -L. (2015). Lewis Y promotes migration of oral cancer cells by glycosylation of epidermal growth factor receptor. PLoS One, 10:e0120162.
  • Ma, C.-Y., Chang, W.-E., Shi, G.-Y., Chang, B.-Y., Cheng, S.-E., Shih, Y.-T., and Wu, H.-L. (2015). Recombinant thrombomodulin inhibits lipopolysaccharide-induced inflammatory response by blocking the functions of CD14. J Immunol, 194:1905-1915.
  • Chao, T. –H., Tsai, W. –C., Chen, J. –Y., Liu, P. –Y., Chung, H. –C., Tseng, S. –Y., Kuo, C. –H., Shi, G. –Y., Wu, H. –L., Li, Y. -H. (2014). Cilostazol enhances mobilization and proliferation of endothelial progenitor cells and collateral formation by modifying vasculo-angiogenic biomarkers in peripheral arterial disease. Int J Cardiol, 172:e371-374.
  • Cheng, T. –L., Lai, C. –H., Jiang, S. –J., Hung, J. –H., Liu, S. –K., Chang, B. –I., Shi, G. –Y., Wu, H. –L. (2014). RHBDL2 is a critical membrane protease for anoikis resistance in human malignant epithelial cells. ScientificWorldJournal, 2014:902987-902995.
  • Cheng, T. –L., Wu, Y. –T., Lai, C. –H., Kao, Y. –C., Kuo, C. –H., Liu, S. –L., Hsu, Y. –Y., Chen, P. –K., Cho, C. –F., Wang, K. –C., Lin, W. –L., Chang, B. –I., Chen C. –M., Hartmut Weiler, Shi, G. –Y., Wu, H. –L. (2013). Thrombomodulin regulates keratinocyte differentiation and promotes wound healing. J Invest Dermatol, 133:1638-1645.
  • Lai, C. –H., Shi, G. –Y., Lee, F. –T., Kuo, C. –H., Cheng, T. –L., Chang, B. –I., Ma, C. –Y., Hsu, F. –C., Yang, Y. –J., Wu, H. –L. (2013). Recombinant human thrombomodulin suppresses calcium chloride-induced abdominal aortic aneurysms in mice. Ann Surg, 258:1103-1110.
  • Chen, P. –K., Chang, B. –I., Kuo, C. – H., Cheng, P. –S., Cho, C. –F., Chang, C. –F., Shi, G. –Y., Wu, H. –L. (2013). Thrombomodulin functions as a plasminogen receptor to modulate angiogenesis. FASEB J, 27:4520-4531.
  • Lin, W. –L., Chang, C. –F., Shi, C. –S., Shi, G. –Y., Wu, H. –L. (2013). Recombinant lectin-like domain of thrombomodulin suppresses vascular inflammation by reducing leukocyte recruitment via interacting with Lewis Y on endothelial cells. Arterioscler Thromb Vasc Biol, 33:2366-2373.
  • Kuo, C. –H., Chen, P. –K., Chang, B. –I., Sung, M. –C., Shi, C. -S., Lee, J. –S., Chang, C. –F., Shi, G. –Y., Wu, H.-L. (2012). The recombinant lectin-like domain of thrombomodulin inhibits angiogenesis through interaction with Lewis Y antigen. Blood, 119:1302-1313.
  • Ma, C. –Y., Shi, G. –Y., Shi, C. -S., Kao, Y. –C., Lin, S. –W., Wu, H -L. (2012). Monocytic thrombomodulin triggers LPS- and Gram-negative bacteria-induced inflammatory response. J Immunol, 188:6328-37.
  • Cheng, T. –L., Wu, Y. –T., Lin H. –Y., Hsu, F. –C., Chang, B. –I., Chen, W. –S., Lai. C. –H., Shi, G. –Y., and Wu, H. –L. (2011). Functions of rhomboid family protease RHBDL2 and thrombomodulin in wound healing. J Invest Derm, 131:2486-2494.
  • Lai, C. -H., Luo, C. -Y., Lin, P. -Y., Kan, C. -D., Chang, R. -S., Wu, H. -L., Yang, Y. -J. (2011). Surgical consideration of in situ prosthetic replacement for primary infected abdominal aortic aneurysms. Eur J Vasc Endovasc Surg, 42:617-624.
  • Wei, H. -J., Li, Y. -H., Shi, G. -Y., Liu, S. -L., Chang, P. -C., Kuo, C. -H., and Wu,H. -L. (2011). Thrombomodulin domains attenuate atherosclerosis by inhibiting thrombin-induced endothelial cell activation. Cardiovasc Res, 92:317-327.
  • He, R.-Y., Lin, C. –Y., Su, Y. –D., Chiu, K. –C., Chang, N. –S., Wu, H. –L., and Chen, S. –J. (2010). Imaging live cell membranes via surface plasmon-enhanced fluorescence and phase microscopy. Opt Exp, 18:3649-3659.
  • Shi, C.-S., Shi, G.-Y., Hsiao, H.-M., Kao, Y.-C., Kuo, K.-L., Ma, C.-Y., Kuo, C.-H., Chang, B.-I., Chang, C.-F., Lin, C.-H., Wong, C.-H., and Wu, H.-L. (2008) Lectin-like domain of thrombomodulin binds to its specific ligand Lewis Y antigen and neutralizes lipopolysaccharide-induced inflammatory response. Blood. 112:3661-3670.
  • Shi, C.-S., Shi, G.-Y., Chang, Y.-S., Han, H.-S., Kuo, C.-H., Liu, C., Huang, H.-C., Chang, Y.-J., Chen, P.-S., and Wu, H.-L. (2005). Evidence of human thrombomodulin domain as a novel angiogenic factor. Circulation. 111:1627-1636.
  • Huang, H.-C., Shi, G.-Y., Jiang, S.-J., Shi, C.-S., Wu, C.-M., Yang, H.-Y., and Wu, H.-L. (2003). Thrombomodulin-mediated cell adhesion: involvement of its lectin-like domain. J Biol Chem. 278:46750-46759.
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